Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay
نویسندگان
چکیده
منابع مشابه
Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.
Amplification of RNA by the polymerase chain reaction (PCR) is normally a two-step process requiring separate enzymes and buffer conditions. We describe a combined reverse transcription-PCR (RT-PCR) assay for hepatitis C virus (HCV) RNA amplification in which a single enzyme and buffer condition are used. In this assay, both the RT and PCR steps are carried out with the thermoactive DNA polymer...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.4.882-886.1993